BamHI is a type II restriction endonuclease enzyme that is commonly used in molecular biology research for DNA manipulation. It is derived from the bacterium Bacillus amyloliquefaciens H and recognizes the DNA sequence 5'-GGATCC-3'. BamHI cleaves DNA at this recognition site, specifically between the G and A bases on both strands, producing cohesive ends with 5' overhangs.
BamHI is used in various molecular biology techniques, such as cloning, restriction mapping, and DNA sequencing. It is often used in combination with other restriction enzymes to generate complementary cohesive ends that can be ligated together.
Overall, BamHI is a valuable tool for DNA manipulation and has been widely used in research labs for many years.
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