What is dispase?

Dispase is a protease enzyme that is commonly used in cell culture to detach or dissociate adherent cells from the cell culture dish or plate. It works by breaking down the proteins that are responsible for cell adhesion, allowing the cells to be easily lifted off the surface.

Dispase is particularly useful for gentle and non-destructive cell dissociation, as it is milder compared to other cell dissociation enzymes such as trypsin. This makes it ideal for maintaining cell viability and integrity during the dissociation process.

Dispase is available in different concentrations and formulations, and the optimal concentration and conditions for use may vary depending on the specific cell type being dissociated. It is important to carefully follow the manufacturer's instructions for the proper use and handling of dispase to ensure optimal results.

Overall, dispase is a valuable tool in cell culture techniques for studying cell behavior, proliferation, and differentiation, and is commonly used in research laboratories and biotechnology companies.