What is immunoelectrophoretic?

Immunoelectrophoresis is a laboratory technique used to separate and identify proteins based on their antigenic properties. It involves the combination of electrophoresis, which separates proteins based on their size and charge, with immunodiffusion, which identifies proteins based on their ability to react with specific antibodies.

During the process of immunoelectrophoresis, a sample containing a mixture of proteins is first separated using electrophoresis. Then, specific antibodies are added to the gel to create immunoprecipitation bands where the protein and antibody meet. The immunoprecipitation bands are then visualized using various staining techniques, such as Coomassie blue staining, to identify and quantify the specific proteins in the sample.

Immunoelectrophoresis is commonly used in clinical laboratories to diagnose and monitor a variety of diseases, such as autoimmune disorders, multiple myeloma, and viral infections. It is also used for research purposes to study protein interactions, protein structure, and protein expression.