What is myc-tagged?

protein expression

Myc-tagged protein expression refers to the insertion of a short peptide tag sequence, derived from the c-myc gene, into a protein of interest. This tag enables the protein to be easily purified and detected using immunochemical methods, such as Western blotting and immunoprecipitation. The Myc tag is often used in protein expression experiments to facilitate the identification and characterization of proteins in in vitro and in vivo studies. The Myc tag typically consists of 10 amino acids (EQKLISEEDL) and is added to the C or N terminus of the target protein. The Myc-tagged protein can be expressed in a variety of expression systems including bacteria, yeast, insect cells, and mammalian cells. The expression level and stability of Myc-tagged proteins depend on the nature of the protein, the protein expression system, and the culture conditions used.