Ouchterlony is a technique used in immunology to detect the presence of antigens or antibodies in a sample. This technique involves the diffusion of antigens and antibodies towards each other in a gel medium, usually agar, to form a visible line of precipitation known as a precipitin line.
In Ouchterlony immunodiffusion, two wells are made in the agar gel, one containing the antigen and the other containing the antibody. As they diffuse towards each other, if the antigen and antibody react with each other, a visible precipitin line will form where they meet.
This technique is commonly used in research labs and diagnostic laboratories to detect the presence of specific antigens or antibodies in samples, such as detecting the presence of a particular protein in a sample, or determining if a patient has developed antibodies against a specific pathogen.
Overall, Ouchterlony immunodiffusion is a valuable tool in immunology for studying antigen-antibody reactions and determining the presence of specific molecules in biological samples.
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