What is nucleofected?

Nucleofection is a technique used to introduce plasmid DNA or siRNA into cells by using an electrical field to create transient pores in the cell membrane. The electrical field created by the nucleofection process generates small gaps in the cell membrane, which allow plasmid DNA or siRNA to be taken up by the cells. The DNA or RNA molecules are then transcribed and translated by the cellular machinery, leading to the expression of the desired protein or suppression of the targeted gene.

Nucleofection is a powerful tool for gene regulation studies and the production of recombinant proteins. This technique is particularly useful for non-dividing or difficult-to-transfect cells, such as primary cells or neurons, which are resistant to traditional transfection methods.

Nucleofection can offer high transfection efficiency with minimal cell damage, resulting in high levels of target gene expression. Different nucleofection settings can be adjusted to achieve optimal transfection efficiency and minimize cellular toxicity. However, nucleofection is more expensive compared to other transfection methods, and it may require specialized equipment and expertise.