What is nucleofector?

Nucleofector is a gene transfer technology that uses an electroporation-based method to deliver nucleic acids such as DNA and siRNA into eukaryotic cells. The technology involves applying short, high-voltage electric pulses to cells to transiently disrupt the cell membrane, allowing the nucleic acid to enter the cell.

The Nucleofector device consists of a generator that delivers predefined electrical pulses, and various cuvette formats that can hold different cell types and volumes. The transfection efficiency and the viability of cells after transfection are typically higher than with other gene transfer methods.

Nucleofection can be used in a wide range of applications, including gene expression analysis, gene knockout, gene editing, and protein production. It has been successfully used in various cell types including primary cells, stem cells, and suspension cells.

Overall, nucleofector is a powerful and versatile tool for transfecting nucleic acids into cells, facilitating a wide range of research applications.